Positional Candidate Cloning of a QTL in Dairy Cattle: Identification of a Missense Mutation in the Bovine DGAT1 Gene with Major Effect on Milk Yield and Composition

Author:

Grisart Bernard,Coppieters Wouter,Farnir Frédéric,Karim Latifa,Ford Christine,Berzi Paulette,Cambisano Nadine,Mni Myriam,Reid Suzanne,Simon Patricia,Spelman Richard,Georges Michel,Snell Russell

Abstract

We recently mapped a quantitative trait locus (QTL) with a major effect on milk composition—particularly fat content—to the centromeric end of bovine chromosome 14. We subsequently exploited linkage disequilibrium to refine the map position of this QTL to a 3-cM chromosome interval bounded by microsatellite markers BULGE13and BULGE09. We herein report the positional candidate cloning of this QTL, involving (1) the construction of a BAC contig spanning the corresponding marker interval, (2) the demonstration that a very strong candidate gene, acylCoA:diacylglycerol acyltransferase (DGAT1), maps to that contig, and (3) the identification of a nonconservative K232A substitution in the DGAT1 gene with a major effect on milk fat content and other milk characteristics.[The sequence data described in this paper have been submitted to the GenBank data library under accession number AY065621.]

Publisher

Cold Spring Harbor Laboratory

Subject

Genetics(clinical),Genetics

Reference28 articles.

1. Genetic dissection of phenotypic diversity in farm animals

2. Boldman K.G. Kriese L.A. Van Vleck L.D. Van Tassel C.P. Kachman S.D. (1995) A manual for use of MTDFREML. A set of programs to obtain estimates of variances and covariances. (U.S. Department of Agriculture, Agriculture Research Service).

3. Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis

4. A QTL with major effect on milk yield and composition maps to bovine Chromosome 14

5. Base-Calling of Automated Sequencer Traces UsingPhred. I. Accuracy Assessment

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