A systematic screen identifies Saf5 as a link between splicing and transcription in fission yeast

Author:

Borao Sonia,Vega Montserrat,Boronat Susanna,Hidalgo Elena,Hümmer Stefan,Ayté José

Abstract

ABSTRACTSplicing is an important step of gene expression regulation in eukaryotes, as there are many mRNA precursors that can be alternatively spliced in different tissues, at different cell cycle phases or under different external stimuli. We have developed several life constructs that allow the quantification of fission yeast splicingin vivoon intact cells, and we have compared their splicing efficiency in a wild type strain and in aprp2-1(U2AF65) genetic background, showing a clear dependency between Prp2 and a consensus signal at 5’ splicing site (5’SS). To isolate novel genes involved in regulated splicing, we have crossed the reporter containing the weakest splicing rate with theSchizosaccharomyces pombeknock out collection. Among the candidate genes involved in the regulation of splicing, we have detected strong splicing defects in two of the mutants (Δcwf12andΔsaf5), both of them related to the NineTeen Complex (NTC). We have identified that strains with mutations incwf12have inefficient splicing, mainly when the 5’SS differs from the consensus. However, althoughΔsaf5cells also have some dependency on 5’SS sequence, we noticed that when one intron of a given pre-mRNA was affected, the rest of the introns of the same pre-mRNA had high probabilities of being also affected. This observation points Saf5 as a link between transcription rate and splicing.

Publisher

Cold Spring Harbor Laboratory

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