Clustered ProtocadherinCis-interactions are Required for Homophilic Combinatorial Cell-Cell Recognition Underlying Neuronal Self-Avoidance

Abstract

AbstractIn the developing human brain, only 53 stochastically expressed clustered protocadherin (cPcdh) isoforms enable neurites from an individual neuron to recognize and self-avoid, while maintaining contact with neurites from other neurons. Cell assays have demonstrated that self-recognition occurs only when all cPcdh isoforms perfectly match across the cell boundary, with a single mismatch in the cPcdh expression profile interfering with recognition. It remains unclear however, how a single mismatched isoform between neighboring cells, is sufficient to block erroneous recognitions. In using systematic cell aggregation experiments we show that abolishing cPcdh interactions on the same membrane (cis) results in a complete loss of specific combinatorial binding between cells (trans). Our computer simulations demonstrate that the organization of cPcdh in linear array oligomers, composed ofcisandtransinteractions, enhances self-recognition by increasing the concentration and stability of cPcdhtranscomplexes between the homotypic membranes. Importantly, we show that the presence of mismatched isoforms between cells drastically diminishes the concentrations and stability of thetranscomplexes. Overall, we provide an explanation for the role of the cPcdh assembly arrangements in neuronal self/non-self-discrimination underlying neuronal self-avoidance.