Alfalfa vein mottling virus, a novel potyvirid infectingMedicago sativaL

Abstract

AbstractWe have recently identified a novel virus detected in alfalfa seed material. The virus was tentatively named alfalfa-associated potyvirus 1, as its genomic fragments bore similarities with potyvirids. In this study, we obtained a complete genome sequence of the virus and classified it as a tentative species in the new genus, most closely related to the members of the genusIpomovirusin the familyPotyviridae. This assumption is based on the genome structure, phylogenetic relationships, transmission electron microscopy investigations and, in part, on serological cross-reactivity of the virus. We also demonstrated its mechanical transmission to the indicator plantNicotiana benthamianaand to the natural hostMedicago sativa, both of which developed characteristic symptoms therefore suggesting a pathogenic nature of the disease. Consistent with symptomatology, the virus was renamed to alfalfa vein mottling virus. A name Alvemovirus was proposed for the new genus in the familyPotyviridae, of which alfalfa vein mottling virus is a tentative member.