Engineering heterothallic strains in fission yeast

Abstract

ABSTRACTIn poor nitrogen conditions, fission yeast cells mate, undergo meiosis and form spores that are resistant to deleterious environments. Natural isolates ofSchizosaccharomyces pombeare homothallic. This allows them to naturally switch between the twoh-andh+mating types with a high frequency, thereby ensuring the presence of both mating partners in a population of cells. However, alteration of the mating type locus can abolish mating type switching or reduce it to a very low frequency. Such heterothallic strains have been isolated and are common in research laboratories due to the simplicity of their use for Mendelian genetics. In addition to the standard laboratory strains, a large collection of naturalS. pombeisolates is now available, representing a powerful resource for investigating the genetic diversity and biology of fission yeast. However, most of these strains are homothallic, and only tedious or mutagenic strategies have been described to obtain heterothallic cells from a homothallic parent. Here, we describe a simple approach to generate heterothallic strains. It takes advantage of an alteration of the mating type locus that was previously identified in a mating type switching-deficient strain and the CRISPR-Cas9 editing tool, allowing for a one-step engineering of heterothallic cells with high efficiency.Take away points-Conventional methods for obtaining heterothallic fission yeast strains are inefficient-We implemented a streamlined genetic editing approach to engineer heterothallism-All fission yeast isolates reported in Jeffares et al. 2015 can be engineered-This method enhances the exploration of genetic diversity in wild fission yeast