Brain-specificGata4downregulation inGreywickfemale mice models the metabolic subtype of polycystic ovary syndrome

Abstract

ABSTRACTPolycystic ovary syndrome (PCOS) is a heterogenous disorder characterized by reproductive and metabolic abnormalities. PCOS etiology remains poorly understood, although the hypothalamus is suspected to play a central role in many cases. Human genetic studies have also shown an association with the transcription factor-coding geneGATA4, but without providing a functional link. Here, we show that adultGreywickfemale mice may bridge this gap. These mice phenocopy PCOS with partial penetrance, due to serendipitous insertion of aGata4promoter-driven transgene in a strong enhancer region. Resulting robust transgene expression in subsets of hypothalamic neurons and glia impairs endogenousGata4expression, resulting in misexpression of genes linked to the control of fertility and food intake. We also show that this previously overlooked role of GATA4 in the hypothalamus can be replicated by conditional knockout approaches. Overall, this study sheds light not only on PCOS etiology but also on the role played by GATA4 in the central control of reproduction.