Single Cell Transcriptome Defines Cell Type Repertoire of AdultDaphnia magna

Abstract

AbstractDetailed knowledge of transcriptional responses to environmental and developmental cues is impossible without single cell (SC) resolution data. We performed two SC RNAseq experiments surveying transcriptional profiles of females and males ofD. magna, a freshwater plankton crustaceanwhichis both a classic and emerging new model for eco-physiology, toxicology, and evolutionary genomics. We were able to identify over 30 distinct cell types about half of which could be functionally annotated. First, we identified ovaries- and testis-related cell types by focusing on female- and male-specific clusters. Second, we compared markers between SC clusters and bulk RNAseq data on transcriptional profiles of early embryos, circulating hemocytes, midgut, heads (containing brain, eyes, muscles and hepatic caeca), antennae II, and carapace. Finally, we compared transcriptional profiles of Daphnia cell clusters with orthologous markers of 250+ cell types annotated in Drosophila cell atlas. This allowed us to recognize striated muscle cells, gut enterocytes, cuticular cells, as well as 5 different neuron types, including photoreceptors and 3 ovaries-related clusters, one of which tentatively identified as the germ line cells. One well-defined cluster showed a significant enrichment in markers of both hemocytes and fat body ofDrosophila, but not with bulk RNAseq data from circulating hemocytes, allowing us to hypothesize the existence of non-circulating, fat body-associated population of hemocytes inDaphnia. On the other hand, the circulating hemocytes express numerous cuticular proteins suggesting their role, in addition to macrophagy, in wound repair. At the same time numerous cell types remain unidentified, including those that map to FCA groups ambiguously or are characterized byDaphnia-specific markers with no clear orthology in the fruitfly. Likewise, many known or presumed cell types or tissues inDaphniahave not been identified to SC clusters. A detailed in-situ hybridization study would be necessary to match not yet annotated SC clusters to functional cell groups.HighlightsFirst single-cell transcriptomic atlas forDaphnia magna, identifies > 30 distinct cell types.Novel cell type representing circulating hemocytes may play a role in cuticle regeneration.Evidence for non-circulating hemocyte-like cells associated with the fat body in Daphnia.Cuticle/epithelial cells expressing photoreceptors, suggesting light-sensing capabilities.Subfunctionalization of divergent paralogs across cell types for ecological versatility.