A replication-competent vesicular stomatitis virus for studies of SARS-CoV-2 spike-mediated cell entry and its inhibition
Author:
Dieterle M. Eugenia, Haslwanter DeniseORCID, Bortz Robert H.ORCID, Wirchnianski Ariel S., Lasso Gorka, Vergnolle Olivia, Abbasi Shawn A., Fels J. Maximilian, Laudermilch EthanORCID, Florez Catalina, Mengotto Amanda, Kimmel Duncan, Malonis Ryan J., Georgiev George, Quiroz Jose, Barnhill Jason, Pirofski Liise-anne, Daily Johanna P., Dye John M., Lai Jonathan R.ORCID, Herbert Andrew S., Chandran KartikORCID, Jangra Rohit K.ORCID
Abstract
SummaryThere is an urgent need for vaccines and therapeutics to prevent and treat COVID-19. Rapid SARS-CoV-2 countermeasure development is contingent on the availability of robust, scalable, and readily deployable surrogate viral assays to screen antiviral humoral responses, and define correlates of immune protection, and to down-select candidate antivirals. Here, we describe a highly infectious recombinant vesicular stomatitis virus bearing the SARS-CoV-2 spike glycoprotein S as its sole entry glycoprotein that closely resembles the authentic agent in its entry-related properties. We show that the neutralizing activities of a large panel of COVID-19 convalescent sera can be assessed in high-throughput fluorescent reporter assay with rVSV-SARS-CoV-2 S and that neutralization of the rVSV and authentic SARS-CoV-2 by spike-specific antibodies in these antisera is highly correlated. Our findings underscore the utility of rVSV-SARS-CoV-2 S for the development of spike-specific vaccines and therapeutics and for mechanistic studies of viral entry and its inhibition.
Publisher
Cold Spring Harbor Laboratory
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