Author:
Banerjee Kushal K.,Saxena Madhurima,Kumar Namit,Chen Lei,Cavazza Alessia,Toke Natalie H.,O'Neill Nicholas K.,Madha Shariq,Jadhav Unmesh,Verzi Michael P.,Shivdasani Ramesh A.
Abstract
After acquiring competence for selected cell fates, embryonic primordia may remain plastic for variable periods before tissue identity is irrevocably determined (commitment). We investigated the chromatin basis for these developmental milestones in mouse endoderm, a tissue with recognizable rostro–caudal patterning and transcription factor (TF)-dependent interim plasticity. Foregut-specific enhancers are as accessible and active in early midgut as in foregut endoderm, and intestinal enhancers and identity are established only after ectopic cis-regulatory elements are decommissioned. Depletion of the intestinal TF CDX2 before this cis element transition stabilizes foregut enhancers, reinforces ectopic transcriptional programs, and hence imposes foregut identities on the midgut. Later in development, as the window of chromatin plasticity elapses, CDX2 depletion weakens intestinal, without strengthening foregut, enhancers. Thus, midgut endoderm is primed for heterologous cell fates, and TFs act on a background of shifting chromatin access to determine intestinal at the expense of foregut identity. Similar principles likely govern other fate commitments.
Funder
National Institutes of Health
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Cited by
33 articles.
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