Author:
Das Susmita,Ranjan Saumya,Keerthana Carmel,Seenivasan Gayathri,Tuti Nikhil,Shaji Unnikrishnan P,Meur Gargi,Anindya Roy
Abstract
AbstractNon-heme iron (FeII) and 2-oxoglutarate(2OG)-dependent dioxygenases catalyse a diverse array of biological reactions. These enzymes couple the oxidative decarboxylation of 2OG to the hydroxylation of the substrates. However, in the absence of the substrate, oxidative decarboxylation of 2OG generates succinate. We have determined succinate level by using succinyl-CoA synthetase to monitor this uncoupled decarboxylation of FeII/2OG-dependent dioxygenases and measured the uncoupled 2OG turnover of different FeII/2OG-dependent dioxygenases. We also performed comparative analysis and verified the functionality of human dioxygenase ALKBH6.
Publisher
Cold Spring Harbor Laboratory
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