Author:
Amit Ido,Yakir Liat,Katz Menachem,Zwang Yaara,Marmor Mina D.,Citri Ami,Shtiegman Keren,Alroy Iris,Tuvia Shmuel,Reiss Yuval,Roubini Eli,Cohen Maya,Wides Ron,Bacharach Eran,Schubert Ullrich,Yarden Yosef
Abstract
The tumor suppressor gene 101 (tsg101) regulates vesicular trafficking processes in yeast and mammals. We report a novel protein, Tal (Tsg101-associated ligase), whose RING finger is necessary for multiple monoubiquitylation of Tsg101. Bivalent binding of Tsg101 to a tandem tetrapeptide motif (PTAP) and to a central region of Tal is essential for Tal-mediated ubiquitylation of Tsg101. By studying endocytosis of the epidermal growth factor receptor and egress of the human immunodeficiency virus, we conclude that Tal regulates a Tsg101-associated complex responsible for the sorting of cargo into cytoplasm-containing vesicles that bud at the multivesicular body and at the plasma membrane.
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Cited by
134 articles.
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