Abstract
SUMMARYThe Coronaviridae is a family of positive-strand RNA viruses that includes SARS-CoV-2, the etiologic agent of the COVID-19 pandemic. Bearing the largest single-stranded RNA genomes in nature, coronaviruses are critically dependent on long-distance RNA-RNA interactions to regulate the viral transcription and replication pathways. Here we experimentally mapped the in vivo RNA-RNA interactome of the full-length SARS-CoV-2 genome and subgenomic mRNAs. We uncovered a network of RNA-RNA interactions spanning tens of thousands of nucleotides. These interactions reveal that the viral genome and subgenomes adopt alternative topologies inside cells, and engage in different interactions with host RNAs. Notably, we discovered a long-range RNA-RNA interaction - the FSE-arch - that encircles the programmed ribosomal frameshifting element. The FSE-arch is conserved in the related MERS-CoV and is under purifying selection. Our findings illuminate RNA structure based mechanisms governing replication, discontinuous transcription, and translation of coronaviruses, and will aid future efforts to develop antiviral strategies.
Publisher
Cold Spring Harbor Laboratory
Reference63 articles.
1. Andrews, R.J. , Peterson, J.M. , Haniff, H.S. , Chen, J. , Williams, C. , Grefe, M. , Disney, M.D. , and Moss, W.N. (2020). An in silico map of the SARS-CoV-2 RNA Structurome. bioRxiv 2020.04.17.045161
2. In Vivo Mapping of Eukaryotic RNA Interactomes Reveals Principles of Higher-Order Organization and Regulation
3. A -1 ribosomal frameshift element that requires base pairing across four kilobases suggests a mechanism of regulating ribosome and replicase traffic on a viral RNA
4. Sequence embedding for fast construction of guide trees for multiple sequence alignment
5. Characterization of an efficient coronavirus ribosomal frameshifting signal: Requirement for an RNA pseudoknot