Tar Spot Disease Severity Influences Phyllosphere-Associated Bacterial and Fungal Microbiomes

Author:

Singh RakshaORCID,Crane Charles F.,Shim Sujoung,Telenko Darcy E. P.,Goodwin Stephen B.ORCID

Abstract

ABSTRACTTar spot, caused by the obligate fungal pathogenPhyllachora maydis, is a foliar disease of corn that has become a major economic concern in the United States. To test the hypothesis thatP. maydiscan interact with other foliar microorganisms, we investigated phyllosphere microbiomes in relation to corn inbreds with differential tar spot symptoms under natural infestation in the field. Leaf samples from sixteen inbred lines were assessed for tar spot symptoms, and bacterial and fungal microbiomes were characterized by paired-end sequencing on the Illumina MiSeq platform. Comparison of the phyllosphere microbiomes revealed distinct bacterial and fungal communities between resistant and susceptible lines. Bacterial and fungal species richness was significantly higher in resistant compared to susceptible inbred lines in a sample-specific manner. In contrast, there were no clear differences in diversity when including evenness of bacterial communities between the resistant and susceptible lines. Diversity of fungal communities differed significantly, particularly between twelve of the fourteen susceptible lines versus resistant lines. Plant-beneficial bacterial genera such asMethylorubrumandQuadrisphaerawere associated with resistant lines, whilePantoea, DeinococcusandPseudomonaswere the least abundant. The second-most commonly detected fungus likely was aConiothyrium, but whether it is the same species that was identified as a possible mycoparasite ofP. maydisin Central and South America is not known. Fungal generaCladosporium, Papiliotrema, Cryptococcus, TilletiopsisandAlternariawere associated with resistant lines whileSphaerellopsiswas the least-abundant genus. In contrast,Puccinia, SphaerellopsisandPhyllachorawere the dominant fungal genera in susceptible lines. Our findings imply thatP. maydisinfection may result in a distinct microbiota with lower diversity. Further analyses of these distinct microbiota between resistant and susceptible lines could lead to a better understanding of the potential role of foliar microbiomes in causing or resistingP. maydisinfection.

Publisher

Cold Spring Harbor Laboratory

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3