Abstract
AbstractBackgroundPolypyrimidine tract binding protein 2 (PTBP2) regulates alternative splicing in neuronal, muscle, and Sertoli cells. PTBP2 and its paralog, PTBP1, which plays a role in B-cell development, was found to be expressed aberrantly in myeloid leukemia.MethodsRNA immunoprecipitation sequencing was used to identify the targets of PTBP2. PTBP2-mediated oxidative phosphorylation and mitochondrial fusion were studied using Seahorse XFp extracellular flux analyzer and confocal microscopy. Athymic nude mice were used to study the role of PTBP2in vivo.ResultsGenetic ablation of Ptbp2 in the cells resulted in decreased cellular proliferation and repopulating ability, decreased ROS, and altered mitochondrial morphology. RNA immunoprecipitation followed by sequencing and functional assays confirmed that PTBP2 binds to Bcl-2 Interacting Protein 3-3’UTR and stabilizes its expression. Our study also suggests that PTBP2 promotes autophagy, as evidenced by the low levels of LC3-II expression in Ptbp2-knockout cells treated with bafilomycin A1. This was restored upon overexpression of Bnip3 in the knockout cells. Finally, we observed that KCL22 cells gave rise to malignant tumors compared to KCL22-Ptbp2 KO cells when injected subcutaneously in the flanks of mice.ConclusionPTBP2 promotes cell proliferation and tumor formation while also enhancing autophagy through Bnip3, thus contributing to the progression of CML.
Publisher
Cold Spring Harbor Laboratory