Escherichia coliextra-intestinal population translocation in leukemia patients

Author:

Marin JulieORCID,Walewski Violaine,Dziri Samira,Magnan Mélanie,Denamur ErickORCID,Carbonnelle Etienne,Bridier-Nahmias AntoineORCID

Abstract

AbstractEscherichia coli, a commensal species of the human gut, is an opportunistic pathogen which can reach extra-intestinal compartments, including the bloodstream and the bladder, among others. In non-immunosuppressed patients, purifying or neutral evolution ofE. colipopulations has been reported in the gut. Conversely, it has been suggested that when migrating to extra-intestinal compartments,E. coligenomes undergo diversifying selection as supported by strong evidence for adaptation. The level of genomic polymorphism and the size of the populations translocating from the gut to extra-intestinal compartments is largely unknown.To gain insights in the pathophysiology of these translocations, we investigated the level of polymorphism and the evolutionary forces acting on the genomes of 77E. coliisolated from various compartments in three immunosuppressed patients. We detected a unique strain for each patient across the blood, the urine and the gut. In one case, all isolates recovered were mutators i.e. isolates with a very high mutation rate. In all instances, we observed that translocation encompasses the majority of the genomic diversity present in the gut. The same signature of selection, whether purifying or diversifying, and as anticipated, neutral for mutator isolates, was observed in both the gut and bloodstream. Additionally, we found a limited number of non-specific mutations among compartments for non-mutator isolates. In all cases, urine isolates were dominated by neutral selection. These findings indicate that substantial proportions of populations are undergoing translocation and that they present a complex compartment-specific pattern of selection at the patient level.ImportanceIt has been suggested that intra and extra-intestinal compartments differentially constrain the evolution ofE. colistrains. Whether host particular conditions, such as immunosuppression, could affect the strain evolutionary trajectories remain understudied. We found that, in immunosuppressed patients, large fractions ofE. coligut populations are translocating with variable modifications of the signature of selection for commensal and pathogenic isolates according to the compartment and/or the patient. Such multiple site sampling should be performed in large cohorts of patients to get a better understanding ofE. coliextra-intestinal diseases.

Publisher

Cold Spring Harbor Laboratory

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