Supercontinuum intrinsic fluorescence imaging heralds ‘free view’ of living systems

Author:

Wang Geng,Li Lianhuang,Liao Xiaoxia,Wang Shu,Mitchell Jennifer,Rabel R.A.C “Chanaka”,Luo Shirui,Milner Derek J.,Wheeler Matthew B.,Gillette Martha U.,Schwing Alexander,Chen Jianxin,Tu Haohua

Abstract

AbstractOptimal imaging strategies remain underdeveloped to maximize information for fluorescence microscopy while minimizing the harm to fragile living systems. Taking hint from the supercontinuum generation in ultrafast laser physics, we generated ‘supercontinuum’ fluorescence from untreated unlabeled live samples before nonlinear photodamage onset. Our imaging achieved high-content cell phenotyping and tissue histology, identified bovine embryo polarization, quantified aging-related stress across cell types and species, demystified embryogenesis before and after implantation, sensed drug cytotoxicity in real-time, scanned brain area for targeted patching, optimized machine learning to track small moving organisms, induced two-photon phototropism of leaf chloroplasts under two-photon photosynthesis, unraveled microscopic origin of autumn colors, and interrogated intestinal microbiome. The results enable a facility-type microscope to freely explore vital molecular biology across life sciences.

Publisher

Cold Spring Harbor Laboratory

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