Abstract
ABSTRACTThe technology of Triplex-forming oligonucleotides (TFOs) provides an approach to manipulate genes at the DNA level. TFOs bind to specific sites on double-stranded DNA, creating a unique triple-helix DNA structure through Hoogsteen hydrogen bonding. This targeting by TFOs is site-specific and the locations TFOs bind are commonly referred to as TFO target sites (TTS). Triplexes have been observed to influence gene expression selectively, stimulate homologous recombination, trigger mutations, inhibit protein binding, and direct DNA damage. These sites typically feature a poly-purine sequence in duplex DNA, and the characteristics of these TTS sequences greatly influence the formation of the triplex. We introduce TTSBBC, a novel analysis and visualization platform designed to explore these features of TTS sequences. The web server can be freely accessed athttps://kowalski-labapps.dellmed.utexas.edu/TTSBBC/.
Publisher
Cold Spring Harbor Laboratory