Author:
Qiu Dawei,Wei Wanyi,Chen Jia,Huang Jingwen,Yang Yong,Luo Ziwei
Abstract
AbstractBone marrow stromal/stem cells (BMSCs) are primitive and heterogeneous cells that can be differentiated into osteoblasts, adipocytes and other subsets. Their bone-fat lineage commitment is responsible for the homeostasis of bone marrow microenvironment. However, there are little effective methods and evidence to simultaneously evaluate the lineage commitment of BMSCs. Here we provide a mixed osteo-adipogenic (OA) differentiation medium that enabled BMSCs differentiation into osteoblasts and adipocytes in vitro, and establish a method for simultaneous assessment of bone-fat lineage choice of BMSCs based on the Alizarin red S and Oil red O staining of specific mineralized nodules and lipid droplets, respectively. This assay provides a specifically simple but effective and low-cost method to visualize the osteogenesis and adipogenesis of BMSCs, and evaluate the efficiency of lineage allocation of BMSCs.Research highlightsA bivalent differentiation medium is prepared for both osteogenic and adipogenic lineage commitment of BMSCs in vitro.A specifically simple but effective and low-cost method is established to simultaneously analyse the efficiency of osteogenesis and adipogenesis of BMSCs.
Publisher
Cold Spring Harbor Laboratory