LIS1 RNA-binding orchestrates the mechanosensitive properties of embryonic stem cells in AGO2-dependent and independent ways

Author:

Kshirsagar AdityaORCID,Gorelik Anna,Olender TsviyaORCID,Sapir TamarORCID,Tsuboi DaisukeORCID,Rosenhek-Goldian IritORCID,Malitsky SergeyORCID,Itkin MaximORCID,Argoetti AmirORCID,Mandel-Gutfreund YaelORCID,Cohen Sidney R.,Hanna JacobORCID,Ulitsky IgorORCID,Kaibuchi KozoORCID,Reiner OrlyORCID

Abstract

AbstractLissencephaly-1 (LIS1) is associated with neurodevelopmental diseases and is known to regulate the activity of the molecular motor cytoplasmic dynein. Here we show that LIS1 is essential for the viability of mouse embryonic stem cells (mESCs), and it regulates the physical properties of these cells. LIS1 dosage substantially affects gene expression, and we uncovered an unexpected interaction of LIS1 with RNA and RNA-binding proteins, most prominently the Argonaute complex. We demonstrate that LIS1 overexpression partially rescued the expression of extracellular matrix (ECM) and mechanosensitive genes conferring stiffness to Argonaute null mESCs. Collectively, our data transforms the current perspective on the roles of LIS1 in post- transcriptional regulation underlying development and mechanosensitive processes.

Publisher

Cold Spring Harbor Laboratory

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. The Emerging Roles ofLIS1 Biomechanics in Cellular and Cortical Homeostasis;Neocortical Neurogenesis in Development and Evolution;2023-08-08

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