Abstract
AbstractAcinetobacter baumanniiis a gram negative nosocomial opportunistic pathogen frequently found in hospital settings, causing high incidence of in-hospital infections. It belongs to the ESKAPE group of pathogens (the “A” stands forA. baumannii) that is known to easily develop antibiotic resistances. It is crucial to create a molecular toolkit to investigate its basic biology, such as gene regulation. DespiteA. baumanniibeing a threat for almost two decades, an efficient and high-throughput plasmid system that can replicate inA. baumanniihas not yet been developed. This study adapts an existing toolkit forEscherichia colito meetA. baumannii’sunique requirements and expands it by constructing a mobile CRISPR interference (CRISPRi) system that can produce gene knockdowns inA. baumannii.
Publisher
Cold Spring Harbor Laboratory
Cited by
1 articles.
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