Egalitarian is a selective RNA-binding protein linking mRNA localization signals to the dynein motor

Author:

Dienstbier Martin,Boehl Florian,Li Xuan,Bullock Simon L.

Abstract

Cytoplasmic sorting of mRNAs by microtubule-based transport is widespread, yet very little is known at the molecular level about how specific transcripts are linked to motor complexes. In Drosophila, minus-end-directed transport of developmentally important transcripts by the dynein motor is mediated by seemingly divergent mRNA elements. Here we provide evidence that direct recognition of these mRNA localization signals is mediated by the Egalitarian (Egl) protein. Egl and the dynein cofactor Bicaudal-D (BicD) are the only proteins from embryonic extracts that are abundantly and specifically enriched on RNA localization signals from transcripts of gurken, hairy, K10, and the I factor retrotransposon. In vitro assays show that, despite lacking a canonical RNA-binding motif, Egl directly recognizes active localization elements. We also reveal a physical interaction between Egl and a conserved domain for cargo recruitment in BicD and present data suggesting that Egl participates selectively in BicD-mediated transport of mRNA in vivo. Our work leads to the first working model for a complete connection between minus-end-directed mRNA localization signals and microtubules and reveals molecular strategies that are likely to be of general relevance for cargo transport by dynein.

Publisher

Cold Spring Harbor Laboratory

Subject

Developmental Biology,Genetics

Reference52 articles.

1. Polypeptide subunits of dynein 1 from sea urchin sperm flagella

2. A conserved 3′→5′ exonuclease active site in prokaryotic and eukaryotic DNA polymerases

3. Black DL Chan R Min H Wang J Bell L (1998) in RNA:protein interactions: A practical approach, The electrophoretic mobility shift assay for RNA binding proteins, ed Smith CWJ (Oxford University Press, Oxford, UK), pp 109–136.

4. Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis

5. The Drosophila hairy RNA localization signal modulates the kinetics of cytoplasmic mRNA transport

Cited by 180 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3