Author:
Julca Irene,Mutwil-Anderwald Daniela,Manoj Vaishnervi,Khan Zahra,Lai Soak Kuan,Yang Lay Kien,Beh Ing Tsyr,Dziekan Jerzy,Lim Yoon Pin,Lim Shen Kiat,Low Yee Wen,Lam Yuen In,Mu Yuguang,Tan Qiao Wen,Nuc Przemyslaw,Choo Le Min,Khew Gillian,Shining Loo,Kam Antony,Tam James P.,Bozdech Zbynek,Schmidt Maximilian,Usadel Bjoern,Kanagasundaram Yoganathan s/o,Alseekh Saleh,Fernie Alisdair,Yeung Li Hoi,Mutwil Marek
Abstract
AbstractNatural products from traditional medicinal plants are valuable candidates for clinical cancer therapy. Plants from the Oldenlandia-Hedyotis complex are popular ingredients of Traditional Chinese Medicine (TCM), however a major hurdle in the plant bioprospecting process of TCM plants is that the active metabolites, their biosynthetic pathways, and mode of action are often unknown. We show that Oldenlandia corymbosa extracts are active against breast cancer cell lines. To study the genes involved in the biosynthesis of active compounds in this medicinal plant, we assembled a high-quality genome. We show that the main active compound is ursolic acid and that abiotic stresses cause changes in anti-cancer activity, metabolite composition, and gene expression of plants. To reveal the mode of action of ursolic acid, we show that cancer cells undergo mitotic catastrophe, and we identify three high-confidence protein binding targets by Cellular Thermal Shift Assay (CETSA) and reverse docking.
Publisher
Cold Spring Harbor Laboratory
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