Cell cycle regulation of the psoriasis associated geneCCHCR1by transcription factor E2F1

Abstract

AbstractThe coiled-coil alpha-helical rod protein 1 (CCHCR1) was first identified as a candidate gene in psoriasis and has lately been associated with COVID-19 susceptibility. Located within P-bodies and centrosomes, its exact cellular role and transcriptional control remain largely unknown. Here, we showed thatCCHCR1shares a bidirectional promoter with its neighboring gene,TCF19. This bidirectional promoter is activated by the G1/S-regulatory transcription factor E2F1, and both genes are co-induced during the G1/S transition of the cell cycle. A luciferase reporter assay suggests that the short intergenic sequence, only 287 bp in length, is sufficient for the G1/S induction of both genes, but the expression ofCCHCR1is further enhanced by the presence of exon 1 from bothTCF19andCCHCR1. This research uncovers the transcriptional regulation of theCCHCR1gene, offering new perspectives on its function. These findings contribute to the broader understanding of diseases associated with CCHCR1 and may serve as a foundational step for future research in these vital medical fields.