Author:
Hu Xinyu,van Sluijs Bob,Blay Óscar García,Stepanov Yury,Rietrae Koen,Huck Wilhelm T.S.,Hansen Maike M. K.
Abstract
AbstractCell fate decisions are ubiquitous and play a critical role throughout development, yet how localization impacts cellular decision making remains unclear. To identify the drivers of position-dependent fate decisions at a molecular level, we developed a scalable antibody and mRNA targeting sequential fluorescence in situhybridization (ARTseq-FISH) method capable of simultaneously profiling mRNAs, proteins and phosphoproteins in single cells at sub-micrometre spatial resolution. We studied 67 unique (phospho-)protein and mRNA targets in individual mouse embryonic stem cells (mESCs) cultured on circular micropatterns, yielding quantification of both abundance and localization of mRNAs and (phospho-)proteins during the first 48 hours of differentiation. ARTseq-FISH revealed a fate decision between continued self-renewal and differentiation that relies solely on the position of each mESC on the micropattern. Our results demonstrate that temporal changes in cell cycle orchestrate these position-dependent cell fate decisions.
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
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