Early mechanisms of aortic failure in a zebrafish model for thoracic aortic dissection and rupture

Abstract

ABSTRACTThoracic aortic aneurysm and dissection (TAAD) associates with a high mortality rate. Despite the existence of different mouse models for TAAD, the underlying disease mechanisms remain elusive. Treatment options are limited and mainly consist of surgical repair at critical aortic diameters as current pharmacological interventions are unable to stop disease progression.In humans, loss of function (LOF) ofSMAD3andSMAD6impairs vascular homeostasis, increasing the risk for TAAD. We developed a zebrafish model for thoracic aortic dissection/rupture by targeting both ohnologs ofsmad3andsmad6. At 10 days post fertilization, we found an increased diameter of the ventral aorta insmad3a−/−;smad3b−/−double knockout zebrafish, whilesmad6a−/−;smad6b−/−double knockout zebrafish have a reduced aortic diameter associated with early mortality. We discovered that asmad3a−/−;smad3b−/−;smad6a−/−;smad6b−/−quadruple knockout (qKO) zebrafish model is viable and survives to adulthood, although exposure to stress leads to sudden death. Histological analysis of the adult ventral aorta shows medial elastolysis, aortic dissections and ruptures at sites exposed to high biomechanical stress. RNA-sequencing of 5 days post fertilization qKO zebrafish indicates a profile of reduced negative regulation of proteolysis and upregulation of melanogenesis, a previously unaddressed pathway in this pathology. We confirm that pharmacological modulation of tyrosinase, the enzyme responsible for the production of melanin, influences aortic morphology.Overall, the qKO mutant, thus far the only known zebrafish model of thoracic aortic dissection and rupture, reveals novel SMAD3/6-dependent pathways that impact thoracic aortic homeostasis, in this way opening avenues for the development of novel treatments in TAAD.