Prolongedin-vivotracking of vitreous fluid and its early diagnostic imaging biomarkers for cancer growth

Abstract

AbstractPurposeEstimation of a correlation between cells in vitreous humour and growth in glioblastoma xenografts.MethodsStreams of cells in vitreous humor are observed in optical coherence tomography (OCT) imaging data of animal (NSGS and Athymic Nude-Foxn1nu) eyes (34 in total) subjected to xenograft growth study, in-vivo. The cancer disease model is studied with and without nanodrug-based treatment protocols.ResultsThe presence of CD8+ and CD4+ is reported inside the tumor using the same data earlier. The transition of these cells is shown to take place from the optic nerve via the vitreous into the nerve fiber layer (NFL) at tumor locations and xenograft -related injuries. Functional analysis of dense temporal imaging series (varying from 28 to more than 100 days) reveals a mild correlation between the volumetric growth of the tumor with the density of these cells, quantitatively and qualitatively.The cross-correlation analysis indicates imaging assisted photodynamic treatment protocol perform relatively better if started with certain delay. Doxorubicin treatment to Nu/Nu Male and NSGS female transforms mild weak negative correlation into mild weak positive correlation.ConclusionsThe plots indicate that the mix of the cells in vitreous humor are effectively dominated by immunosuppressor cytotoxic component.Translational RelevanceWe propose that the vitreous cell density can be used as imaging biomarker helpful for clinicians in early diagnosis and treatment planning of similar disease models. The limitation of this work is that high resolution OCT systems and data-dependent image segmentation methods are required.