Using design of experiments to guide genetic optimization of engineered metabolic pathways

Author:

Moon Seonyun12,Saboe Anna2,Smanski Michael J12ORCID

Affiliation:

1. Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota , St Paul , MN 55108, USA

2. Biotechnology Institute, University of Minnesota , St Paul , MN 55108, USA

Abstract

Abstract   Design of experiments (DoE) is a term used to describe the application of statistical approaches to interrogate the impact of many variables on the performance of a multivariate system. It is commonly used for process optimization in fields such as chemical engineering and material science. Recent advances in the ability to quantitatively control the expression of genes in biological systems open up the possibility to apply DoE for genetic optimization. In this review targeted to genetic and metabolic engineers, we introduce several approaches in DoE at a high level and describe instances wherein these were applied to interrogate or optimize engineered genetic systems. We discuss the challenges of applying DoE and propose strategies to mitigate these challenges. One-Sentence Summary This is a review of literature related to applying Design of Experiments for genetic optimization.

Funder

University of Minnesota

Publisher

Oxford University Press (OUP)

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