Use of Organic Acids as a Model to Study the Impact of Gut Microflora on Nutrition and Metabolism

Author:

Dibner J.J.,Buttin P.

Publisher

Elsevier BV

Subject

Animal Science and Zoology

Reference69 articles.

1. Alimet Feed Supplement. Alimet is a trademark of Novus International, Inc., St. Charles, MO, and is registered in the United States and other countries.

2. Acidification advantage of analogue methionine;Buttin;Int. Pig Topcs,1999

3. In vitro study on the effect of organic acids on Campylobacter jejuni/coli populations in mixtures of water and feed;Chaveerach;Poult. Sci.,2002

4. Escherichia coli was obtained from the American Type Culture collection (#25922). It was grown in sterile trypticase soy broth (TSB) containing HCl, 2-hydroxy-4-(methylthio) butanoic acid (HMB, Alimet), formic acid, or lactic acid at 1%. Acidity of the TSB was adjusted with HCl or NaOH to 4 or 7.3. Cultures were initiated with E. coli stock culture that had been grown in TSB, centrifuged, and resuspended in 0.1% peptone water to 107 cfu/mL. Bacteria (100 µL) were inoculated into test medium and incubated 35°C with agitation. Samples were removed from each treatment after 5 and 24 h of incubation. Samples were serially diluted and plated on trypticase soy agar for enumeration. All plates were incubated at 35°C for 24 h. Populations of E. coli were reported as colony-forming units per milliliter.

5. Organic acids for performance enhancement in pig diets;Partanen;Nutr. Res. Rev.,1999

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