Post-natal developmental changes in the composition of the rat neocortical N-glycome

Author:

Klarić Thomas S1ORCID,Salopek Matija1,Micek Vedran2,Gornik Kljaić Olga1,Lauc Gordan13ORCID

Affiliation:

1. Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia

2. Laboratory Animals Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia

3. Genos Glycoscience Research Laboratory, Zagreb, Croatia

Abstract

Abstract Asparagine-linked glycosylation (N-glycosylation) plays a key role in many neurodevelopmental processes, including neural cell adhesion, neurite outgrowth and axon targeting. However, little is known about the dynamics of N-glycosylation during brain development and, in particular, how the N-glycome of the developing neocortex differs from that of the adult. The aim of this study, therefore, was to perform a thorough characterization of N-glycosylation in both the adult and neonatal rat neocortex in order to gain insights into the types of changes occurring in the N-glycome during neurodevelopment. To this end, we used hydrophilic interaction ultraperformance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry to compare the adult neocortical N-glycome with that of 24- and 48-h neonates. We report that the abundance of complex N-glycans is significantly lower in adults compared with neonates. Furthermore, the proportion of charged complex N-glycans is also greatly reduced. This decrease in the abundance of complex N-glycans is offset by a corresponding increase in the proportion of truncated and, to a lesser extent, hybrid N-glycans. Lastly, we report that although the proportion of oligomannose N-glycans remains constant at around 24%, the distribution of high-mannose subtypes shifts from predominantly large subtypes in neonates to smaller subtypes in the adult. In summary, our findings indicate that N-glycan synthesis in the rat neocortex is fundamentally different in neonates compared with adults with a general shift occurring from large, sialylated N-glycans towards smaller, neutral structures as neonates develop into adults, coupled with a parallel shift towards smaller oligomannose structures.

Funder

Croatian National Centre of Research

Centre of Competence in Molecular Diagnostics

Genos Glycoscience Research Laboratory

Publisher

Oxford University Press (OUP)

Subject

Biochemistry

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