A Hepatocyte Nuclear FactorBtabHNF4Mediates Desiccation Tolerance and Fecundity in Whitefly (Bemisia tabaci)

Author:

Wang Kui1,Yang Zhifang1,Li Xiang1,Liu Shunxiao12,Wang Liuhao1,Zhang Hongwei1,Yu Hao1ORCID

Affiliation:

1. Department of Natural Resources, Henan Institute of Science and Technology , Xinxiang 453003, Henan Province , China

2. College of Agrarian Technology and Natural Resources, Sumy National Agrarian University , Sumy 40021 , Ukraine

Abstract

AbstractHepatocyte nuclear factor 4 (HNF4) is essential for glucose homeostasis and lipid metabolism in insects. However, little is known about the role of HNF4 in whiteflies. In the present study, we identified a hepatocyte nuclear factor protein from Bemsia tabaci (Diptera: Drosophilidae) and named it BtabHNF4. The full-length of BtabHNF4 was 3,006 bp, encoding a sequence of 434 amino acids that contains a conserved zinc-finger DNA-binding domain (DBD) and a well-conserved ligand-binding domain (LBD). The temporal and spatial expression showed that BtabHNF4 was highly expressed in the female adult stage and abdominal tissues of B. tabaci. A leaf-mediated RNA interference method was used to explore the function of BtabHNF4 in whiteflies. Our results showed that the knockdown of BtabHNF4 influences the desiccation tolerance, egg production, and egg hatching rate of whiteflies. Additionally, BtabHNF4 silencing significantly inhibited the expression level of vitellogenin. These results expand the function of HNF4 and pave the way for understanding the molecular mechanisms of HNF4 in regulating multiple physiological processes.

Funder

National Natural Science Foundation of China

Science and Technology Project of Henan Province

Initiating Foundation of Henan Institute of Sciences and Technology

Publisher

Oxford University Press (OUP)

Subject

Insect Science,Ecology,Ecology, Evolution, Behavior and Systematics

Reference56 articles.

1. The Sf1-related nuclear hormone receptor Hr39 regulates Drosophila female reproductive tract development and function.;Allen,2008

2. dHNF4 regulates lipid homeostasis and oogenesis in Drosophila melanogaster;Almeida-Oliveira;Insect Biochem. Mol. Biol,2021

3. Retinoic acid-induced apoptosis in leukemia cells is mediated by paracrine action of tumor-selective death ligand TRAIL;Altucci;Nat. Med,2001

4. Posttranslational modifications of lipid-activated nuclear receptors: focus on metabolism;Becares;Endocrinology,2017

5. Multidomain integration in the structure of the HNF-4α nuclear receptor complex;Chandra;Nature,2013

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3