Phylogenetic patterns and ontogenetic origins of limb length variation in ecologically diverse lacertine lizards

Abstract

Abstract Limb length is intrinsically linked to function and, ultimately, fitness. Thus, it can co-evolve with habitat structure, as exemplified by tropical lizards in highly heterogeneous environments. But does lizard limb length respond in a similar manner during adaptive diversification in temperate zones? Here, we examine variation in habitat preference and limb length in lacertine lizards from the Palaearctic. We tested the following three hypotheses: (1) species of the Lacertini tribe descended from a generalist ancestor and subsequently underwent habitat specialization; (2) specialized ecological roles are associated with relative limb length in extant species; and (3) interspecific differences in limb length emerge in embryonic development. Our comparisons supported an ancestral ‘rocky’ or ‘generalist’ habitat preference, and phenotype–habitat associations were particularly supported when examining size-adjusted forelimb length in 69 species that represented all known Lacertini genera. Moreover, we revealed an elevated interlimb ratio in high-vegetation species, which might be linked to climbing performance in species with relatively longer forelimbs. Furthermore, embryonic limb variation was detected solely against an Eremiadini outgroup species. Instead, hind limb length differences within Lacertini originated in post-hatching ontogeny. The mechanisms that modulate limb growth are likely to be limited in Lacertini, because adaptive morphological change might mirror historical contingency and the ecological context wherein this clade diversified.