Unveiling novel Neocosmospora species from Thai mangroves as potent biocontrol agents against Colletotrichum species

Author:

Klomchit Anthikan1,Calabon Mark S2,Worabandit Sompradtana1,Weaver Jack A3,Karima Elfina M4,Alberti Fabrizio3,Greco Claudio4ORCID,Mahanil Siraprapa1ORCID

Affiliation:

1. School of Science, Mae Fah Luang University , Chiang Rai 57100 , Thailand

2. Division of Biological Sciences, College of Arts and Sciences, University of the Philippines Visayas , Miagao, Iloilo 5024 , Philippines

3. School of Life Sciences, University of Warwick , Gibbet Hill Road, Coventry CV4 7AL , United Kingdom

4. Department of Biosciences, Swansea University , Swansea SA2 8PP , United Kingdom

Abstract

Abstract Aims Neocosmospora species are saprobes, endophytes, and pathogens belonging to the family Nectriaceae. This study aims to investigate the taxonomy, biosynthetic potential, and application of three newly isolated Neocosmospora species from mangrove habitats in the southern part of Thailand using phylogeny, bioactivity screening, genome sequencing, and bioinformatics analysis. Methods and results Detailed descriptions, illustrations, and a multi-locus phylogenetic tree with large subunit ribosomal DNA (LSU), internal transcribed spacer (ITS), translation elongation factor 1-alpha (ef1-α), and RNA polymerase II second largest subunit (RPB2) regions showing the placement of three fungal strains, MFLUCC 17–0253, MFLUCC 17–0257, and MFLUCC 17–0259 clustered within the Neocosmospora clade with strong statistical support. Fungal crude extracts of the new species N. mangrovei MFLUCC 17–0253 exhibited strong antifungal activity to control Colletotrichum truncatum CG-0064, while N. ferruginea MFLUCC 17–0259 exhibited only moderate antifungal activity toward C. acutatum CC-0036. Thus, N. mangrovei MFLUCC 17–0253 was sequenced by Oxford nanopore technology. The bioinformatics analysis revealed that 49.17 Mb genome of this fungus harbors 41 potential biosynthetic gene clusters. Conclusion Two fungal isolates of Neocosmospora and a new species of N. mangrovei were reported in this study. These fungal strains showed activity against pathogenic fungi causing anthracnose in chili. In addition, full genome sequencing and bioinformatics analysis of N. mangrovei MFLUCC 17–0253 were obtained.

Funder

BBSRC

EPSRC

Swansea University

Engineering and Physical Sciences Research Council

UKRI

Mae Fah Luang University

Publisher

Oxford University Press (OUP)

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