Integrative immunophenotypic and genetic characterization of acute myeloid leukemia with CBFB rearrangement

Author:

Sameeta Fnu1,Wang Sa A1,Tang Zhenya1,Khoury Joseph D2,Fang Hong1,Wang Dylan1,Xu Jie1,Li Shaoying1,Hu Zhihong3,Hu Shimin1,Jorgensen Jeffrey L1,Medeiros L Jeffrey1,Wang Wei1

Affiliation:

1. Department of Hematopathology, The University of Texas MD Anderson Cancer Center , Houston, TX , US

2. Department of Pathology and Microbiology, University of Nebraska Medical Center , Omaha, NE , US

3. Department of Laboratory Medicine, The University of Texas MD Anderson Cancer Center , Houston, TX , US

Abstract

Abstract Objectives We sought to characterize the immunophenotype of acute myeloid leukemia (AML) with CBFB rearrangement and correlate the results with cytogenetic and molecular data. Methods Sixty-one cases of AML with CBFB rearrangement were evaluated. Results The sample population consisted of 33 men and 28 women, with a median age of 49 years. Flow cytometry immunophenotypic analysis showed that myeloblasts were positive for CD34 and CD117 in all cases, and myeloperoxidase was positive in 52 of 55 (95%) cases. The most common abnormalities included decreased CD38 in 90%, increased CD13 in 85%, increased CD123 in 84%, and decreased HLA-DR in 84% of cases. Monocytes were increased, with a mature immunophenotype, and accounted for 23.7% of total cells. Among 60 cases with available karyotype, inv(16)(p13.1q22) was most common in 50 (83%) cases, followed by t(16;16) (p13.1;q22) in 6 (10%). Type A CBFB::MYH11 transcript was most common, detected in 84% of cases. Mutational analysis showed mutations of NRAS in 37%, FLT3 in 25%, and KIT in 24% of cases. Comparing cases with type A vs non–type A transcripts, blasts in type A cases more frequently exhibited CD64 positivity and increased CD13 levels while showing a lower frequency of CD7 and CD56 expression. Trisomy 22 and mutations in KIT, NF1, and TET2 were identified only in cases with type A transcript. Conclusions Myeloblasts of AML with CBFB rearrangement are positive for CD34, CD117, and myeloperoxidase. These neoplasms most frequently carry inv(16)(p13.1q22) and type A fusion transcript. NRAS mutation was the most common mutation. Some immunophenotypic and genetic correlations occurred with different types of transcripts.

Publisher

Oxford University Press (OUP)

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