Distribution and role of d-glutamate, a novel d-amino acid identified in animals, in the reproductive tissues of male kuruma prawn Marsupenaeus japonicus

Author:

Yoshikawa Naoko1,Yoshitomi Natsuki1,Nakada Kazuki1,Sawada Naomi1

Affiliation:

1. Shizuoka Institute of Science and Technology Department of Materials and Life Science, Faculty of Science and Technology, , Fukuroi, Shizuoka 437-8555, Japan

Abstract

Abstract Some aquatic invertebrates contain free d-alanine. We previously showed copious amounts of free d-glutamate, a novel d-amino acid, in the tissue of the male reproductive organs of Marsupenaeus japonicus. Herein, we clarified the distribution and potential role of d-glutamate and d-alanine in male reproductive tissues, namely the testis, vas deferens and seminal receptacle at different growth stages of M. japonicus. The percentage of d-glutamate to total glutamate was over 50% in these tissues. In particular, the content of d-glutamate was the most abundant in the vas deferens, the ratio of d-glutamate to total glutamate was approximately 80%. In contrast, d-alanine content was the lowest in the vas deferens among these tissues. d-Glutamate content was the highest when the prawn weighed 12 g, indicating that d-glutamate is actively synthesized in the younger stage. Our findings suggest that d-glutamate plays an important role in the reproductive functions of M. japonicus.

Funder

Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan

Publisher

Oxford University Press (OUP)

Subject

Molecular Biology,Biochemistry,General Medicine

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