Measurable impact of RNA quality on gene expression results from quantitative PCR

Author:

Vermeulen Joëlle,De Preter Katleen,Lefever Steve,Nuytens Justine,De Vloed Fanny,Derveaux Stefaan,Hellemans Jan,Speleman Frank,Vandesompele Jo

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference27 articles.

1. Detection of rare mRNAs via quantitative RT-PCR;Weis;Trends Genet.,1992

2. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays;Bustin;J. Mol. Endocrinol.,2000

3. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream;Ginzinger;Exp. Hematol.,2002

4. Real-time quantitative PCR – opportunities and pitfalls;Bustin;Eur. Pharm. Rev.,2008

5. Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard? Expert Rev;Murphy;Mol. Diagn.,2009

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