The Inability of Marburg Virus to Cause Disease in Ferrets Is Not Solely Linked to the Virus Glycoprotein

Author:

Schiffman Zachary12ORCID,Garnett Lauren12,Tran Kaylie N1,Cao Wenguang1,He Shihua1,Emeterio Karla1,Tierney Kevin1,Azaransky Kim1,Strong James E123,Banadyga Logan12ORCID

Affiliation:

1. Special Pathogens Program, National Microbiology Laboratory Branch, Public Health Agency of Canada , Winnipeg, Manitoba , Canada

2. Department of Medical Microbiology and Infectious Diseases, University of Manitoba , Winnipeg, Manitoba , Canada

3. Department of Pediatrics and Child Health, University of Manitoba , Winnipeg, Manitoba , Canada

Abstract

Abstract Ebola virus (EBOV) causes lethal disease in ferrets, whereas Marburg virus (MARV) does not. To investigate this difference, we first evaluated viral entry by infecting ferret spleen cells with vesicular stomatitis viruses pseudotyped with either MARV or EBOV glycoprotein (GP). Both viruses were capable of infecting ferret spleen cells, suggesting that lack of disease is not due to a block in MARV entry. Next, we evaluated replication kinetics of authentic MARV and EBOV in ferret cell lines and demonstrated that, unlike EBOV, MARV was only capable of low levels of replication. Finally, we inoculated ferrets with a recombinant EBOV expressing MARV GP in place of EBOV GP. Infection resulted in uniformly lethal disease within 7–9 days postinfection, while MARV-inoculated animals survived until study endpoint. Together these data suggest that the inability of MARV to cause disease in ferrets is not entirely linked to GP.

Funder

Public Health Agency of Canada

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

Reference14 articles.

1. The ferret as a model for filovirus pathogenesis and countermeasure evaluation;Schiffman;ILAR J,2021

2. The domestic ferret (Mustela putorius furo) as a lethal infection model for 3 species of ebolavirus;Cross;J Infect Dis,2016

3. Ferrets infected with bundibugyo virus or Ebola virus recapitulate important aspects of human filovirus disease;Kozak;J Virol,2016

4. Comparative transcriptomics in Ebola Makona-infected ferrets, nonhuman primates, and humans;Cross;J Infect Dis,2018

5. The Makona variant of Ebola virus is highly lethal to immunocompromised mice and immunocompetent ferrets;Wong;J Infect Dis,2018

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3