Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection

Author:

Rydell Gustaf E1ORCID,Prakash Kasthuri1,Larsson Simon B1,Skoglund Catarina23,Ringlander Johan1ORCID,Andersson Maria E1,Castedal Maria23,Norder Heléne1,Lindh Magnus1

Affiliation:

1. Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

2. The Transplant Institute, Department of Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden

3. The Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden

Abstract

Abstract Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated strongly when replication was high, supporting that transcription was from covalently closed circular DNA (cccDNA). By contrast, enhanced expression of S RNA relative to cccDNA and core RNA in patients with medium-high or low replication supports that HBV surface antigen (HBsAg) can be expressed mainly from integrated HBV DNA in such patients.

Funder

Swedish Cancer Society

Swedish Government

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

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