Scalp bacterial species influence <i>SIRT1</i> and <i>TERT</i> expression in keratinocytes-Reference-Cited by-同舟云学术

Scalp bacterial species influence SIRT1 and TERT expression in keratinocytes

Published:2023-09-06 Issue:11 Volume:87 Page:1364-1372
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Yamada Azusa¹,Watanabe Kota²,Nishi Yuri¹,Oshiro Mugihito¹,Katakura Yoshinori³,Sakai Kenji¹⁴,Tashiro Yukihiro¹⁴^ORCID

Affiliation:

1. Laboratory of Soil and Environmental Microbiology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University , Fukuoka , Japan

2. Laboratory of Fermentation Microbiology, Department of Fermentation Science, Faculty of Applied Biosciences, Tokyo University of Agriculture , Tokyo , Japan

3. Laboratory of Cellular Regulation Technology, Division of Systems Bioengineering, Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University , Japan

4. Laboratory of Microbial Environmental Protection, Tropical Microbiology Unit, Center for International Education and Research of Agriculture, Faculty of Agriculture, Kyushu University , Fukuoka , Japan

Abstract

ABSTRACT Scalp bacteria on the human scalp and scalp hair comprise distinct community structures for sites and individuals. To evaluate their effect on human keratinocyte cellular activity, including that of the hair follicular keratinocytes, the expression of several longevity genes was examined using HaCaT cells. A screening system that uses enhanced green fluorescent protein (EGFP) fluorescence was established to identify scalp bacteria that enhance silent mating type information regulation 2 homolog-1 (SIRT1) promoter activity in transformed HaCaT cells (SIRT1p-EGFP). The results of quantitative polymerase chain reaction revealed that several predominant scalp bacteria enhanced (Cutibacterium acnes and Pseudomonas lini) and repressed (Staphylococcus epidermidis) the expressions of SIRT1 and telomerase reverse transcriptase (TERT) genes in HaCaT cells. These results suggest that the predominant scalp bacteria are related to the health of the scalp and hair, including repair of the damaged scalp and hair growth, by regulating gene expression in keratinocytes.

Funder

JSPS

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbad122/51666550/zbad122.pdf

Reference51 articles.

1. The human skin microbiome;Byrd;Nat Rev Microbiol,2018

2. SIRT1 and aging related signaling pathways;Chen;Mech Ageing Dev,2020

3. Protection against UVB-induced photoaging by Nypa fruticans via inhibition of MAPK/AP-1/MMP-1 signaling;Choi;Oxid Med Cell Longev,2020

4. TERT promotes epithelial proliferation through transcriptional control of a Myc- and Wnt-related developmental program;Choi;PLoS Genet,2008

5. Mushroom extract inhibits ultraviolet B-induced cellular senescence in human keratinocytes;Chong;Cytotechnology,2018