SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling-Reference-Cited by-同舟云学术

SELEX-discovered aptamer that inhibits cellular interleukin-17/interleukin-17 receptor interaction and antagonizes interleukin-17 signaling

Published:2023-11-29 Issue:2 Volume:88 Page:147-153
ISSN:1347-6947
Container-title:Bioscience, Biotechnology, and Biochemistry
language:en
Short-container-title:

Author:

Munshi Arifur Rahman¹,Wang Tong¹,Takamori Yukio¹,Ando Takehiro¹,Yokoyama Takumi¹,Fuji Daisuke¹,Xu Zhehao²,Vedi Santhana¹,Yamamoto Mizuki¹,Tsukamoto Keita²,Kawakami Takashi³⁴^ORCID

Affiliation:

1. Department of Integrated Applied Life Science, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi , Kofu, Yamanashi , Japan

2. Department of Life and Environmental Sciences, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi , Kofu, Yamanashi , Japan

3. Faculty of Life and Environmental Sciences, Graduate Faculty of Interdisciplinary Research, University of Yamanashi , Kofu, Yamanashi , Japan

4. JST, PRESTO , Kawaguchi, Saitama , Japan

Abstract

ABSTRACT This research is based on a Systematic Evolution of Ligands by EXponential enrichment, also referred to as in vitro selection against the extracellular domain of human interleukin-17 receptor A (IL-17RA). Pull-down assay via quantitative polymerase chain reaction and chemiluminescence detection showed that the cloned RNA with the enriched sequence bound to human IL-17RA and inhibited the interaction between IL-17RA and human interleukin-17A (IL-17A). We also revealed that the newly discovered IL-17RA-binding RNA aptamer bound to cellular IL-17RA, inhibited the cellular IL-17RA/IL-17A interaction, and antagonized cellular IL-17A signaling.

Funder

JSPS

Heiwa Nakajima Foundation

JST

PRESTO

Publisher

Oxford University Press (OUP)

Subject

Organic Chemistry,Molecular Biology,Applied Microbiology and Biotechnology,General Medicine,Biochemistry,Analytical Chemistry,Biotechnology

Link

https://academic.oup.com/bbb/advance-article-pdf/doi/10.1093/bbb/zbad170/54878055/zbad170.pdf

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