Our Growing Volume Electron Microscopy Community

Author:

Czymmek Kirk J1ORCID,Darrow Michele C2,Verkade Paul3,Ortega-Sandoval Mario E3,Wacker Kate1

Affiliation:

1. Advanced Bioimaging Laboratory, The Donald Danforth Plant Science Center , Saint Louis, MO, 63132

2. The Rosalind Franklin Institute, Harwell Campus , Didcot, Oxfordshire, OX11 0QX, Great Britain

3. School of Biochemistry, University of Bristol , Bristol, BS8 1TH, Great Britain

Abstract

Abstract Volume electron microscopy (vEM) represents a powerful group of electron microscopy workflows that enable collection of large three-dimensional volumes at ultrastructural resolution to interrogate cells and tissues across length scales. Due to the breadth of approaches, users are challenged with numerous specialized and nuanced workflows and the requirements for handling, segmenting, visualizing, and quantifying large and complex datasets. As such, a vEM community of core facilities, early adopters, and commercial vendors of vEM solutions have come together to reduce the barriers for successful adoption of vEM. Supported by the Chan Zuckerberg Initiative, the rapidly expanding community has built a broad coalition sharing knowledge and working together to accelerate improvements in results, speed, and automation in vEM workflows. Here we provide an overview of the vEM concept, our growing resources, and an open invitation to join our community and upcoming vEM Technology Forum with technical talks, discussions, hands-on demonstrations, and networking.

Publisher

Oxford University Press (OUP)

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