The effect of acyl-CoA synthetase long-chain family member 5 on triglyceride synthesis in bovine preadipocytes
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Published:2019-05-06
Issue:1
Volume:62
Page:257-264
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Yu Xiang, Fang Xibi, Xiao Hang, Zhao Zhihui, Maak Steffen, Wang Mengyan, Yang RunjunORCID
Abstract
Abstract. Acyl-CoA synthetase long-chain family member 5 (ACSL5)
is a member of the acyl coenzyme A (CoA) long-chain synthase families (ACSLs), and it
plays a key role in fatty acid metabolism. In this study, we proved an association
between the ACSL5 gene and triglyceride metabolism at the cellular
level in cattle. pBI-CMV3-ACSL5 and pGPU6/GFP/Neo-ACSL5 plasmids were
constructed and transfected into bovine preadipocytes by electroporation. The expression
level of ACSL5 was detected by real-time quantitative PCR and western blot. The
triglyceride content was detected by a triglyceride kit. The results indicated that the
expression level of ACSL5 mRNA and protein in the
pBI-CMV3-ACSL5-transfected group was significantly increased compared with those
in the control group. Furthermore, the pGPU6/GFP/Neo-ACSL5-transfected group was
significantly decreased compared with those in the control group. A cell triglyceride
test showed that overexpression or silencing of the ACSL5 gene could affect
synthesis of cellular triglycerides. This study investigated the mechanism of ACSL on
bovine fat deposition, and also provides a new candidate gene for meat quality traits in
beef cattle.
Publisher
Copernicus GmbH
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