Novel MHC BLB2 gene polymorphism and its association with IgY concentration and Newcastle disease antibody titer in IPB-D2 chickens
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Published:2023-09-21
Issue:3
Volume:66
Page:275-283
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ISSN:2363-9822
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Container-title:Archives Animal Breeding
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language:en
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Short-container-title:Arch. Anim. Breed.
Author:
Lestari Dwi,Murtini Sri,Ulupi Niken,Gunawan Asep,Sumantri Cece
Abstract
Abstract. This study aimed to identify the polymorphism of the B Locus Beta 2
(BLB2) gene and its association with immunoglobulin Y (IgY) concentration and
Newcastle disease (ND) antibody titer; we analyzed BLB2 gene expression in
different categories of ND antibody titers in IPB-D2 chickens. The total
sample used was 100 IPB-D2 chickens. Blood samples were collected at 21
weeks old for an ELISA (enzyme-linked immunoassay) test, an HI (hemagglutination inhibition) test, and genotyping. The method for BLB2
polymorphism was Sanger sequencing. Analysis of BLB2 gene expression was performed using
the cecal tonsil tissue of IPB-D2 chickens. Polymorphism data were analyzed
using SNPstats and DNAsp (DNA Sequence Polymorphism) software. The association of the single-nucleotide
polymorphisms (SNPs) with IgY concentration and ND antibody titer was
analyzed using SAS software (version 9.2). The genotype mean values were compared by means of a
T test. The relative mRNA expression analysis was performed using a quantitative real-time polymerase chain reaction (qRT-PCR). The results
showed that 13 SNPs were found in exon 2 and exon 3 in the BLB2 gene. As many as 4
out of the 13 SNPs were associated with IgY concentration. As many as 9 out the 13
SNPs may have changed amino acids. The ΔCt value showed that the
expression of the BLB2 gene in IPB-D2 chickens with high ND antibody titers is
higher than IPB-D2 chickens with low ND antibody titers. In conclusion, the AA
genotype of g.458 T > A was associated with high IgY concentrations,
and the BLB2 gene presented with a high expression in IPB-D2 chickens with high ND antibody
titers.
Funder
Kementerian Riset, Teknologi dan Pendidikan Tinggi
Publisher
Copernicus GmbH
Reference35 articles.
1. Abumrad, N. N., and Barbul, A.: The Use of Arginine In Clinical Practice In Metabolic & Therapeutic Aspects of Amino Acids Clinical Nutrition, CRC Press, Boca Raton, Florida, 595–611, 2004. 2. Allendorf, F. W., Luikart, G., and Aitken, S. N.: Conservation and The Genetics of Populations, Willey-Blackwell, New York, 225 United State, ISBN 978-0-470-67145-0 2012. 3. Chen, F., Pan, L., Chao, W., Dai, Y., and Yu, W.: Character of chicken
polymorphic major histocompability complex class II alleles of 3 Chinese
local breeds, Poult. Sci., 91, 1097–1104, 2012. 4. Da Silva, D. W. and Tambourgi, D. V.: IgY: a promosing antibody for use in
immunodiagnostic and in immunotherapy, Vet. Immunol. Immunopathol.,
135, 173–180, 2010. 5. Guo, X. L., Zheng, H., Li, X. L., Gu, Z. L., Zheng, C. S., Wei, Z. H., Wang,
J. S., Zhou, R. Y., and Li, L. H.: Genetic variation of major
histocompability complex BLB2 gene exon 2 in Hebei domestic chicken,
Res. Vet., 92, 76–79, 2012.
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