Author:
Zimin Andrei A.,Karmanova Alexandra N.,Lu Yinhua
Abstract
Metagenomic approaches provide access to the genetic diversity of the environment for biotechnological applications, allowing the discovery of new enzymes and new pathways for numerous catalytic processes. Five new putative type II CRISPR-Cas9 DNA endonucleases were identified from the compost community using the DELTA-BLAST algorithm. It was determined using phylogenetic UPGMA analysis that four of these potential enzymes are similar to those of the Bacteroidetes. Protein structural modeling confirmed the data of DELTA-BLAST and UPGMA analysis. These new five proteins found may be promising for genome editing in termoresistant Actinomyces.
Reference14 articles.
1. Metagenomic analysis of microbial consortia enriched from compost: new insights into the role of Actinobacteria in lignocellulose decomposition
2. Karmanova A.N., Zimin A.A., J. of Physics: Conference Series, 1701 (2020),
3. NCBI:taxid702656:https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=702656
4. Impact of Nitriles on Bacterial Communities
5. Kumar S., Stecher G., Li M., Knyaz C., Tamura K., Molecular Biology and Evolution, 35.(2018).
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