Author:
Yasa Ngurah Sedana,Purnomo Sugeng Joko,Anshory Lutfi,Murwantoko ,Isnansetyo Alim,Handayani Niken Satuti Nur
Abstract
Prokaryotic and eukaryotic organisms contain heat shock proteins (HSPs), crucial for rapid response to environmental stress. However, their specific roles in different stress conditions are not fully understood. This study investigated HSP70 and HSP90 expression in H. diversicolor squamata, using qRT-PCR. Data analysis employed SPSS, including t-tests and ANOVA, with significance set at P < 0.05. Results showed distinct expression patterns of HSP genes under varying TSS levels. Both HSP70 and HSP90 mRNA levels significantly increased in response to TSS stressors, with HSP70 exhibiting the highest sensitivity to TSS changes. The duration and amount of TSS exposure influenced gene transcripts, particularly notable at 12 hours and 150 mgL-1 concentration. These findings suggest HSP genes play a role in cellular stress responses to environmental stimuli. HSP70 and HSP90, sensitive to TSS stress, can serve as biomarkers for assessing stress levels from TSS exposure and detecting TSS contamination in abalone farming.
Reference48 articles.
1. Water quality and sludge characterization at raceway-system trout farms
2. Membrane biological reactor treatment of a saline backwash flow from a recirculating aquaculture system
3. Wetzel R.G., 2001. Limnology: Lake and River Ecosystems, 3rd edition. (Academic Press, San Diego, 2001).
4. Timmons M.B., Ebeling J.M., Wheaton F.W., Summerfelt S.T., Vinci B.J., Recirculating Aquaculture Systems 2nd Ed. (Cayuga Aqua Ventures, Ithaca,, 2002).
5. Toxicity of ammonia to three marine fish and three marine invertebrates