Evaluation of Various Silicon- and Boron-Containing Compounds for the Detection of Phosphorylation in Peptides via Gas-Phase Ion-Molecule Reactions

Author:

Piatkivskyi Andrii1,Pyatkivskyy Yuriy12,Ryzhov Victor1

Affiliation:

1. Department of Chemistry and Biochemistry, Northern Illinois University, DeKalb, IL 60115

2. Current address: Waters Corporation 34 Maple St, Milford, MA 01757

Abstract

Gas-phase ion–molecule reactions (IMR) of various boron- and silicon-containing neutrals were investigated as a potential route for detecting phosphorylation within peptides in the negative ion mode. Trimethyl borate (TMB), triethyl borate (TEB) and N,O-Bis(trimethylsilyl)acetamide (TMSA), unlike diethylmethoxyborane (DEMB), diisopropoxymethylborane (DiPMB) and chlorotrimethylsilane (TMSCl), reacted differently if a phosphate moiety was present and thus are suitable to detect phosphorylation. During multistage collision-induced dissociation experiments of the reaction products of IMR with TMB and TEB, the [LSsF − 4H + B] ion formed a modified y2* fragment allowing the phosphorylation site to be assigned, unlike reaction products of DEMB and DiPMB which lost both the phosphoric acid and the boron-containing moiety.

Publisher

SAGE Publications

Subject

Spectroscopy,Atomic and Molecular Physics, and Optics,General Medicine

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