Abstract
Endogenous opioid system plays an important role in the regulation of body functions under stress, providing stress-protective, analgesic and immunoregulatory effects. The aim of this work was to assess the effect of acute cold stress on the in vivo production of adaptive immunity cytokines IL-2, IL-4, IFNγ, phagocytosis, and production of reactive oxygen species in non-immunized mice with induced blockage of opioid receptors. The object of the study were male white mice subjected to acute cold stress at -20 °C for 10 or 60 minutes. To block opioid receptors, naloxone hydrochloride was used, which was administered subcutaneously at a dose of 0.2 mg/kg 20 min before inducing the stress. After the cold exposure, spleen and peritoneal lavage were obtained from the animals. The cytokine concentrations were determined using ELISA technique. The absorption activity of CD11+ cells of the peritoneal cavity was assessed using FITC-stained St.cohnii with a flow cytometer; the production of reactive oxygen species was assessed using the reaction of luminol-dependent chemiluminescence.
It was found that the both cold stress regimens caused naloxone-independent inhibition of spontaneous IFNγ production. In stimulated cultures, an inhibitory effect on IFNγ secretion was registered in animals subjected to stress for only 60 min, being also independent on the opioid receptor blockade. IL-2 production decreased in stimulated cultures against the background of 60 min stress naloxone independently. Both variants of cold stress had no effect on IL-4 production. Stress for 60 min inhibited absorption activity of CD11+ cells from the peritoneal lavage and activated production of oxygen radicals, being, however, canceled by naloxone administration. Hence, acute cold stress led to naloxone-independent inhibition of Th1 cytokine production by splenocytes, naloxone-dependent inhibition of phagocytosis and activation of the microbicidal potential of peritoneal cavity cells.
Publisher
Russian Society of Immunology
Reference19 articles.
1. Гейн С.В., Баева Т.А., Гейн О.Н., Черешнев В.А. Роль моноцитов в реализации эффектов β-эндорфина и селективных агонистов µ- и δ-опиатных рецепторов на пролиферативную активность лимфоцитов периферической крови // Физиология человека, 2006. Т. 32, № 3. С 111-116. [Gein S.V., Baeva T.A., Gein O.N., Chereshnev V.A. The role of monocytes in the effects of beta-endorphin and selective agonists of mu- and delta-Opiate receptors on the proliferative activity of peripheral blood lymphocytes. Fiziologiya cheloveka = Human Physiology, 2006, Vol. 32, no. 3, pp. 111-116. (In Russ.)]
2. Гейн С.В., Брагина Н.А., Шаравьева И.Л. Влияние стресса на антителогенез, продукцию IL-2, IL-4, IFN-γ в зависимости от времени введения антигена и оценка роли опиоидных рецепторов // Бюллетень экспериментальной биологии и медицины, 2023. Т. 175. № 3. С. 298-304. [Gein S.V., Bragina N.A., Sharav’eva I.L. Effect of stress on the production of antibodies and IL-2, IL-4, IFNγ depending on the time of antigen administration and evaluation of the role of opioid receptors. Byulleten eksperimentalnoy biologii i meditsiny = Bulletin of Experimental Biology and Medicine, 2023, Vol. 175, no. 3, pp. 298-304. (In Russ.)]
3. Гейн С.В., Кадочникова Я.А. Влияние эндоморфинов-1,2 на функциональную активность нейтрофилов и моноцитов периферической крови in vitro // Физиология человека, 2021. Т. 47, № 6. С. 65-71. [Gein S.V., Kadochnikova Y.A. Effect of endomorphins-1, 2 on functional activity of neutrophils and peripheral blood monocytes in vitro. Fiziologiya cheloveka = Human Physiology, 2021, Vol. 47, no. 6, pp. 65-71. (In Russ.)]
4. Гейн С.В., Шаравьева И.Л. Влияние холодового стресса на функциональную активность перитонеальных макрофагов мыши в условиях блокады опиатных рецепторов // Российский физиологический журнал им. Сеченова, 2016. Т. 102, № 2. С. 188-194. [Gein S.V., and Sharav’eva I.L. Effects of cold stress on the functional activity of mouse peritoneal macrophages in conditions of opiate receptor. Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova = I. Sechenov Russian Physiological Journal, 2016, Vol. 102, no. 2, pp. 188-194. (In Russ.)]
5. Макарова О.В., Трунова Г.В., Диатроптов М.Е., Серебряков С.Н., Кондашевская М.В., Малайцев В.В. Сравнительная характеристика продукции цитокинов активированными конканавалином А спленоцитами мышей BALB/C и С57В1/6 при холодовом воздействии // Бюллетень экспериментальной биологии и медицины, 2005. Т. 139, № 2. С. 188- 190. [Makarova. O.V., Trunova G.V., Diatroptov M.E., Serebryakov S.N., Kondashevskaya M.V., Malaitsev V.V. Comparative characterization of cytokine production by concanavalin A-activated splenocytes from BALB/c and C57BL/6 mice after cold exposure. Byulleten eksperimentalnoy biologii i meditsiny = Bulletin of Experimental Biology and Medicine, 2005, Vol. 139, no. 2, pp. 188-190. (In Russ.)]