OPTIMIZATION OF LACTOBACILLUS PLANTARUM FERMENTATION FOR ENHANCED PHENOLIC PRODUCTION FROM EXTRACT OF BAJAKAH BARK (UNCARIA NERVOSA) BY RESPONSE SURFACE METHODOLOGY

Abstract

Objective: This research was purposed to optimize the fermentation medium and condition of Lactobacillus plantarum to enhance the production of phenolic compounds with the antibacterial activity tested against Propionibacterium acnes and the optimum conditions of bajakah bark fermentation using Response Surface Method (RSM) methods. Methods: The antibacterial activity test ethanolic extract of bajakah bark was assayed using the agar diffusion perforator method and fermentation method using L. plantarum. Measurement of phenolic content fermentation and without fermentation was done using the standard methods with gallic acid as standard. The optimum conditions of bajakah bark fermentation using RSM methods using Software Design Expert 10.0.0.1. Results: As the result, Response surface analysis revealed the optimum values of the tested significant variables for the production of phenolic were extracted in a concentration 40% (w/v) and 30% (w/v) sucrose for 3 d fermentation period. Under this optimal condition, the phenolic compounds were improved from 10.66 to 18.46 mg GAE/g extract. As well as the antibacterial activity of the fermented extract was increased by 1.35 times compared to the non-fermented extract. Conclusion: In summary that, the optimized fermentation condition could be helpful for the production of antibacterial metabolites from bajakah bark by L. plantarum.