SPECTROPHOTOMETRIC QUANTIFICATION OF GLICLAZIDE IN PHARMACEUTICAL DOSAGE FORM

Abstract

Objective: A simple and sensitive spectrophotometric method has been proposed for quantification of Gliclazide in pure and dosage form. Methods: A visible spectrophotometric methods based on the ion-pair formation of drug with the dye Cresol Red (Method A) and Bromophenol Blue (Method B) in methanol medium. Results: A purple colour complex was formed in Method A while orange colour was obtained by Method B with maximum absorption at 510 nm and 445 nm, respectively. The Beers law was found linear over the concentration range from 30 to 200µg/ml (Method A) and from 70 to 230µg/ml (Method B) with good correlation coefficients 0.9918 and 0.9916 respectively. The molar extinction coefficient were 0.1326 x 104 L/mol/cm (Method A) and 0.1294 x 104L/mol/cm (Method B), LOD 0.2563, 0.4681 and LOQ 0.6394µg/ml and 0.9256 µg/ml. Conclusion: The proposed methods were statistically evaluated for accuracy, precision and linearity in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed methods can be applied in routine analytical and quality control laboratories for the quantification of Gliclazide. The methods are simple, sensitive, rapid and economical for the estimation of Gliclazide in pure and dosage forms.