COMPREHENSIVE MOLECULAR STUDY REVEALS THE POTENTIAL ROLE OF CHEBULINIC ACID AND BOERAVINONE B TO ESTABLISH REDOX HOMEOSTASIS IN METABOLICALLY STRESSED CELLS

Abstract

Objective: Our objective was to assess the multi-dimensional protective mechanism of Chebulinic acid (CA) and Boeravinone B (BB) against MG-induced metabolic stress and cytotoxicity. Methods: In this study, we have studied CA-and BB-mediated changes in molecular markers of highly dynamic mechanisms of mitochondrial disintegration, nuclear damage and cell death pathways associated with early ageing in MG-exposed Saccharomyces cerevisiae cells using biochemical assays, qRT-PCR and flow cytometry. Results: We found that CA and BB interventions during MG-induced stress in S. cerevisiae reduce the rate of extracellular nitrite production, protein carbonyl content, lipid peroxidation and in addition, photo components positively modulate the expression patterns of genes involved in different cell death pathways. Furthermore, CA and BB treatments to MG-stressed cells reduced the number of cells in late apoptosis by 13.4% and 28.3%, respectively. On top of that, CA and BB supplementation during MG-stress restored mitochondrial membrane potential (ΔΨm) by 63.0% and 62.5%, respectively. Conclusion: Based on the results of this study, it seems CA and BB phytotherapy protect against MG-induced cytotoxicity through their natural antioxidant properties by establishing redox homeostasis; thus, CA and BB defend the cell components from oxidative damage of different biomolecules and organelles, ultimately increase longevity.