BIOCHEMICAL EVALUATION OF INDUS VIVA I PULSE NATURAL AYURVEDIC SYRUP AND IT’S IN SILICO – INTERACTION ANALYSIS

Abstract

Objectives: In developing countries like India, diabetes mellitus and human bone cancer are progressively increasing which is global encumbrance. The objective of the study is to find out the phytoconstituents of Indus Viva I Pulse health drink extract that has undergone classical chemical characterization, molecular docking, anti-diabetic, anticancer, antibacterial and antifungal studies used as traditional and complementary medicine. The study aimed to evaluate the Gas Chromatography–Mass Spectrometry (GC-MS), High-Performance Liquid Chromatography (HPLC), Liquid Chromatography–High Resolution Mass Spectrometry (LC-HRMS), anticancer, antidiabetic and spectral studies that are used to disclose phytochemicals and their medicinal properties. Methods: Infrared (IR), Ultraviolet (UV), 1H-NMR spectroscopic methods, GC-MS, HPLC and LC-HRMS instrumental detections were employed. Molecular docking, anti-diabetic, anticancer, antibacterial and antifungal studies were carried out using AutoDock 4.2.6. software program, α- Amylase method, Mosmann and Disk diffusion method respectively. Results: GC-MS and HPLC chromatogram of methanol extracts exhibited 12 and 6 peaks, respectively, confirming the presence of 12 phytoconstituents in Indus Viva I Pulse health drink (1I–12I). The total phenol and flavonoid content found in the extracted sample of Indus Viva I Pulse health drink were 0.16 and 0.36 mg/mL, respectively. It is a polyherbal formulation of brown-colored liquid of the UV spectrum in methanol is characteristic of an aromatic compound with λ max of 319 and 412 nm. IR spectrum gave peaks at 3,457, 2,922, 2,857, 2,121, 1,641, 1,055, 1,033 and 621 cm-1 indicating the presence of an alcoholic, alkyl, aldehyde or ketone, alkyne, carbonyl, anhydride and methyl group, respectively. Liquid Chromatography- High Resolution Mass Spectrometry (LC-HRMS) study gave these molecular weights (m/z): 1134. 7017, 1150.6475 and 1168.6797. Aromatic characteristics were confirmed through UV, IR, 1H-NMR, 13C-NMR and LC-HRMS spectral studies. 1H-NMR study indicates the presence of aromatic protons and methyl protons. Aqueous extract of Indus Viva I Pulse health drink was tested for Cytotoxicity Assay of human bone cancer (MG-63) cell lines and ptyalin prevent characters that exhibited a valid inhibitory value of 90.87% in 10 μg/mL for MG-63 and value of 74.41% in 500 μg/mL for alpha-amylase which is comparatively more efficient than the available standard drug. Cytotoxicity assay for human bone cancer (MG-63) cell lines and alpha-amylase both showed the inhibitory activity of IC50 68.95 and IC50 36.18 μg/mL, respectively. Conclusion: Based on the result of this research, it can be proposed that Indus Viva I Pulse health drink may serve as a potential remedy for human bone cancer (MG-63) cell line and that alpha-amylase inhibitory activity is proportional to the dose.